Determination of Organophosphorus Pesticides in Soybean by Gas Chromatography

  • WenLiang Cai
Keywords: Organophosphorus Pesticides, Gas Chromatography, Purification Reagent, Soybean Detection


Organophosphorus pesticides can effectively control agricultural pests, improving the yield and quality of crops.
However, the problem of pesticide residues is becoming more and more serious due to the extensive use of
pesticides; potential teratogenicity and carcinogenicity pose a serious threat to consumers' health. In order to
control the pollution of organophosphorus pesticide residues, besides strengthening the management of pesticide
use, the key is to develop simple, fast and sensitive detection technology. Because soybean contains more oil
and fat than cereals, it brings many difficulties to the analysis of pesticides. So far, there is no mature method in
China. Based on the above background, the aim of this paper is to determine organophosphorus pesticides in
soybean by gas chromatography. In this paper, the extract ant and sample purifier of organic phosphorus in
soybean were discussed, and the organophosphorus pesticides in soybean were extracted with acetonedichloromethane
mixed solvent, and Flori silica was used as purifying agent. The residues of four
organophosphorus pesticides in soybean, dimethoate, methyl parathion and parathion, were determined by gas
chromatography with flame photometric detector. Simple operation, high recovery and minimum detection
concentration are as follows: dropping fear 0.03 mg/kg; dimethoate 0.04 mg/kg; methyl parathion 0.04 mg/kg;
parathion 0.05 mg/kg.