Determination of Peanut Allergen Protein in Food by Double Antibody Sandwich ELISA

  • Chao Li
Keywords: Double Antibody Sandwich Method, Peanut Allergen, Food Allergy, Polyclonal Antibody


Allergic diseases, such as allergic asthma and food allergies, are common and frequently-occurring diseases in
the clinic. They are listed by the World Health Organization as one of the three major diseases to be prevented
and controlled in the 21st century 10% ~ 30%. Among them, peanut as a major food allergen can cause severe
food allergic reactions including anaphylactic shock and allergic death. The purpose of this article is to
determine the peanut allergen protein content in food to solve the problem that different allergic patients may
respond to different peanut allergen protein components. The double antibody sandwich ELISA method is used
to determine the peanut allergen protein component in food. Detection of peanut allergens in imported and
exported foods and prevention of food allergic diseases caused by peanuts provide a technical basis. By
extracting total peanut protein, immunizing mice to prepare a polyclonal antibody against peanut total protein,
coating the enzyme-labeled plate with the antibody, and labeling the polyclonal antibody with biotin, thereby
establishing a double-poly antibody sandwich ELISA method, and then making peanut total protein The
standard product was tested for the sensitivity of the method, and at the same time, 15 kinds of foods were tested
for the content of peanut protein. The research results show that the developed dual-antibody sandwich ELISA
method has specificity for detecting peanut allergen protein components in food, and the minimum detection
limit is 8ng / mL, and the standard curve is linear in the range of 8ng / mL ~ 125 ng / mL; The test results of
13 kinds of foods are consistent with the contents of the food allergen label, and the test results of 2 kinds of
unknown foods are all positive.